So that you are interested in getting the largest signal possible (at the maxima) in order to have the best sensitivity with the set of given concentration, which you cannot increase of decrease infinitely due to limited solubility and device limitations – most spectrophotometers won't register $A > 2$ properly. Where $y$ – signal (absorbance) $a$ – background noise or erroneous values (can be often taken 0 for a dual-beam spectrometer with a blank probe) $x$ – concentration. Sensitivity $b$ is the slope of your linear calibration curve: (1) The sensitivity of the analysis is greatest at maximum absorbance that is, we get the maximum response for a given concentration of analyte. ![]() ![]() Yes, it does, and especially for calibration curves :įor spectrophotometric analysis, we normally choose the wavelength of maximum absorbance for two reasons:
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